Scientific Publications

Comparisons of the antibody repertoires of a humanized rodent and humans by high throughput sequencing

Collin Joyce, Dennis R. Burton, Bryan Briney Scientific Reports, 2020

Sequence-Based Discovery Demonstrates That Fixed Light Chain Human Transgenic Rats Produce a Diverse Repertoire of Antigen-Specific Antibodies.

Harris KE, Force Aldred S, Davison LM, Ogana HA, Boudreau A, Brüggemann M, Osborn M, Ma B, Buelow B, Clarke SC, Dang KH, Iyer S, Jorgensen B, Pham DT, Pratap PP, Rangaswamy US, Schellenberger U, van Schooten WC, Ugamraj HS, Vafa O, Buelow R, Trinklein ND. Front. Immunol., (2018); 9: 889.

Bacterially derived synthetic mimetics of mammalian oligomannose prime antibody responses that neutralize HIV infectivity.

Pantophlet R, Trattnig N, Murrell S, Lu N, Dennis Chau D, Rempel C, Wilson IA, Kosma P. Nature Communications (2017) 8: 1601

Strategies to Obtain Diverse and Specific Human Monoclonal Antibodies From Transgenic Animals.

Brüggemann M, Osborn MJ, Ma B, Buelow R. Transplantation (2017) 101(8):1770-1776.

Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies.

Ouisse LH, Gautreau-Rolland L, Devilder MC, Osborn M, Moyon M, Visentin J, Halary F, Bruggemann M , Buelow R, Anegon I, Saulquin X. BMC Biotechnology (2017) 17:3

Human Antibody Production in Transgenic Animals.

Brüggemann M, Osborn MJ, Ma B, Hayre J, Avis S , Lundstrom B, Buelow R. Arch. Immunol. Ther. Exp. (2015) 63:101–108

Transgenic Animals Derived by DNA Microinjection.

Brüggemann M, Osborn MJ, Ma B, Avis S, Anegon I, Buelow R. Handbook of Therapeutic Antibodies ( 2014) pp77-88

Human antibody expression in transgenic rats: Comparison of chimeric IgH loci with human VH, D and JH but bearing different rat C-gene regions.

Ma B, Osborn MJ, Avis S, Ouisse L-H, Ménoret S, Anegon I, Buelow R, Brüggemann M. Journal of Immunological Methods (2013) 400–401 (2013) 78–86.

High-Affinity IgG Antibodies Develop Naturally in Ig-Knockout Rats Carrying Germline Human IgH/Igk/Igl Loci Bearing the Rat CH Region.

Osborn MJ, Ma B, Avis S, Binnie A, Dilley J, Yang X, Lindquist K, Ménoret S, Iscache A-L Ouisse L-H, Rajpal A, Anegon I,Neuberger MS, Buelow R, Brüggemann M. J Immunol. (2013) 90(4):1481-90.

Characterization of immunoglobulin heavy chain knockout rats.

Ménoret S, Iscache AL, Tesson L, Rémy S, Usal C, Osborn MJ, Cost GJ, Brüggemann M, Buelow R, Anegon I. Eur. J. Immunol. (2010). 40: 2932–2941.

Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases.

Geurts AM, Cost GJ, Freyvert Y, Zeitler B, Miller JC, Choi VM, Jenkins SS, Wood A, Cui X, Meng X, Vincent A, Lam S, Michalkiewicz M, Schilling R, Foeckler J, Kalloway S, Weiler H,1,2 Ménoret S, Anegon I, Davis GD, Zhang L, Rebar EJ,3Gregory PD, Urnov FD, Jacob HJ, Buelow R. Science, (2009) 325(5939), 433–433.

Discovery of high affinity, pan-allelic, and pan- mammalian reactive antibodies against the myeloid checkpoint receptor SIRPα

Janet Sim, Jonathan T. Sockolosky, Emma Sangalang, Shelley Izquierdo,Darlene Pedersen, William Harriman, Ardian S. Wibowo, Josh Carter, Anup Madan, Laura Doyle,Ons Harrabi, Steven E. Kauder, Amy Chen, Tracy C. Kuo, Hong Wan & Jaume Pons MAbs. 2019 Aug/Sep;11(6):1036-1052. doi: 10.1080/19420862.2019.1624123. Epub 2019 Jul 1.

V(D)J Rearrangement Is Dispensable for Producing CDR-H3 Sequence Diversity in a Gene Converting Species.

Leighton PA, Morales J, Harriman W, Ching KH. Front Immunol. (2018) 11; 9:1317.

A multiplatform strategy for the discovery of conventional monoclonal antibodies that inhibit the voltage-gated potassium channel Kv1.3.

Bednenko J, Harriman R, Mariën L, Nguyen HM, Agrawal A, Papoyan A, Bisharyan Y, Cardarelli J, Cassidy-Hanley D, Clark T, Pedersen D, Abdiche YN, Harriman W, van der Woning B, de Haard H, Collarini E, Wuldd H, Colussi P. MAbs. (2018) 10(4):636-650.

Chickens with humanized immunoglobulin genes generate antibodies with high affinity and broad epitope coverage to conserved targets.

Ching KH, Collarini EJ, Abdiche YN, Bedinger D, Pedersen D, Izquierdo S, Harriman R, Zhu L, Etches RJ, van de Lavoir MC, Harriman WD, Leighton PA. MAbs. (2018) 10(1):71-80.

Generation of a highly diverse panel of antagonistic chicken monoclonal antibodies against the GIP receptor.

Könitzer JD, Pramanick S, Pan Q, Augustin R, Bandholtz S, Harriman W, Izquierdo S. MAbs. (2017) 9(3):536-549.

High-efficiency antibody discovery achieved with multiplexed microscopy.

Mettler Izquierdo S, Varela S, Park M, Collarini E, Lu D, Pramanick S, Rucker J, Lopalco L, Etches R & Harriman W. Microscopy (Oxf). (2016) 65(4):341-52.

Assessing kinetic and epitopic diversity across orthogonal monoclonal antibody generation platforms.

Abdiche YN, Harriman R, Deng X, Yeung YA, Miles A, Morishige W, Boustany L, Zhu L, Mettler-Izquierdo S & Harriman W. MAbs. (2015) 8(2):264-77.

A Diverse Repertoire of Human Immunoglobulin Variable Genes in a Chicken B Cell Line is Generated by Both Gene Conversion and Somatic Hypermutation.

Leighton PA, Schusser B, Yi H, Glanville J & Harriman W. Front Immunol. (2015) 20;6:126

Harnessing gene conversion in chicken B cells to create a human antibody sequence repertoire.

Schusser B, Yi H, Collarini EJ, Izquierdo SM, Harriman WD, Etches RJ, Leighton PA. PLoS One. (2013) 21;8(11):e80108.

Expression of human lambda expands the repertoire of OmniChickens

Ching KH, Berg K, Morales J, Pedersen D, Harriman WD, Abdiche YN, Leighton PA. PLoS One. 2020 Jan 29;15(1):e0228164. doi: 10.1371/journal.pone.0228164. eCollection 2020.

Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes.

Collarini, E. J, Leighton PA, van de Lavoir M-C. Methods Mol Biol. (2019) 1874:403-430

Germline Gene Editing in Chickens by Efficient CRISPR-Mediated Homologous Recombination in Primordial Germ cells.

Dimitrov L, Pedersen D, Ching KH, Yi H, Collarini EJ, Izquierdo S, Van De Lavoir MC, Leighton PA. PLoS ONE, (2016) 11(4): e0154303.

Generation of chickens expressing Cre recombinase.

Leighton PA, Pedersen D, Ching K, Izquierdo S, Collarini EJ, Jacob R and van de Lavoir MC. Transgenic Research (2016) 25(5), 609–616.

Expression of heavy chain-only antibodies can support B-cell development in light chain knockout chickens.

Schusser B, Collarini E, Pedersen D, Yi H, Ching K, Izquierdo S, Thoma T, Lettmann S, Kaspers B, Etches RJ, van de Lavoir MC, Harriman W, Leighton PA. 2016. Eur J Immunol. (2016) 46(9):2137-48.

Inserting random and site-specific changes into the genome of chickens.

Collarini E, Leighton P, Pedersen D, Harriman B, Jacob R, Mettler-Izquierdo S, Yi H, Van De Lavoir MC & Etches RJ. Poult Sci. (2015) 94(4):799-803.

Immunoglobulin knockout chickens via efficient homologous recombination in primordial germ cells.

Schusser B, Collarini EJ, Yi H, Izquierdo SM, Fesler J, Pedersen D, Klasing KC, Kaspers B, Harriman WD, van de Lavoir MC, Etches RJ & Leighton PA. Proc Natl Acad Sci USA. (2013) 110(50):20170-5.

Common light chain chickens produce human antibodies of high affinity and broad epitope coverage for the engineering of bispecifics

Kathryn H. Ching, Kimberley Berg, Kevin Reynolds, Darlene Pedersen, William D. Harriman & Philip A. Leighton, 2021