OmniRat/Omnimouse

• Harris KE, Force Aldred S, Davison LM, Ogana HA, Boudreau A, Brüggemann M, Osborn M, Ma B, Buelow B, Clarke SC, Dang KH, Iyer S, Jorgensen B, Pham DT, Pratap PP, Rangaswamy US, Schellenberger U, van Schooten WC, Ugamraj HS, Vafa O, Buelow R, Trinklein ND. Sequence-Based Discovery Demonstrates That Fixed Light Chain Human Transgenic Rats Produce a Diverse Repertoire of Antigen-Specific Antibodies. Front. Immunol., (2018); 9: 889.

• Pantophlet R, Trattnig N, Murrell S, Lu N, Dennis Chau D, Rempel C, Wilson IA, Kosma P. Bacterially derived synthetic mimetics of mammalian oligomannose prime antibody responses that neutralize HIV infectivity. Nature Communications (2017) 8: 1601

• Brüggemann M, Osborn MJ, Ma B, Buelow R. Strategies to Obtain Diverse and Specific Human Monoclonal Antibodies From Transgenic Animals. Transplantation (2017) 101(8):1770-1776.

• Ouisse LH, Gautreau-Rolland L, Devilder MC, Osborn M, Moyon M, Visentin J, Halary F, Bruggemann M , Buelow R, Anegon I, Saulquin X. Antigen-specific single B cell sorting and expression-cloning from immunoglobulin humanized rats: a rapid and versatile method for the generation of high affinity and discriminative human monoclonal antibodies. BMC Biotechnology (2017) 17:3

• Brüggemann M, Osborn MJ, Ma B, Hayre J, Avis S , Lundstrom B, Buelow R. Human Antibody Production in Transgenic Animals. Arch. Immunol. Ther. Exp. (2015) 63:101–108

• Brüggemann M, Osborn MJ, Ma B, Avis S, Anegon I, Buelow R. Transgenic Animals Derived by DNA Microinjection. Handbook of Therapeutic Antibodies ( 2014) pp77-88

• Ma B, Osborn MJ, Avis S, Ouisse L-H, Ménoret S, Anegon I, Buelow R, Brüggemann M. Human antibody expression in transgenic rats: Comparison of chimeric IgH loci with human VH, D and JH but bearing different rat C-gene regions. Journal of Immunological Methods (2013) 400–401 (2013) 78–86.

• Osborn MJ, Ma B, Avis S, Binnie A, Dilley J, Yang X, Lindquist K, Ménoret S, Iscache A-L Ouisse L-H, Rajpal A, Anegon I,Neuberger MS, Buelow R, Brüggemann M. High-Affinity IgG Antibodies Develop Naturally in Ig-Knockout Rats Carrying Germline Human IgH/Igk/Igl Loci Bearing the Rat CH Region. J Immunol. (2013) 90(4):1481-90.

• Ménoret S, Iscache AL, Tesson L, Rémy S, Usal C, Osborn MJ, Cost GJ, Brüggemann M, Buelow R, Anegon I. Characterization of immunoglobulin heavy chain knockout rats. Eur. J. Immunol. (2010). 40: 2932–2941.

• Geurts AM, Cost GJ, Freyvert Y, Zeitler B, Miller JC, Choi VM, Jenkins SS, Wood A, Cui X, Meng X, Vincent A, Lam S, Michalkiewicz M, Schilling R, Foeckler J, Kalloway S, Weiler H,1,2 Ménoret S, Anegon I, Davis GD, Zhang L, Rebar EJ,3Gregory PD, Urnov FD, Jacob HJ, Buelow R. Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases. Science, (2009) 325(5939), 433–433.

Antibody Discovery-OmniChicken

• Leighton PA, Morales J, Harriman W, Ching KH. V(D)J Rearrangement Is Dispensable for Producing CDR-H3 Sequence Diversity in a Gene Converting Species. Front Immunol. (2018) 11; 9:1317.

• Bednenko J, Harriman R, Mariën L, Nguyen HM, Agrawal A, Papoyan A, Bisharyan Y, Cardarelli J, Cassidy-Hanley D, Clark T, Pedersen D, Abdiche YN, Harriman W, van der Woning B, de Haard H, Collarini E, Wuldd H, Colussi P. A multiplatform strategy for the discovery of conventional monoclonal antibodies that inhibit the voltage-gated potassium channel Kv1.3. MAbs. (2018) 10(4):636-650.

• Ching KH, Collarini EJ, Abdiche YN, Bedinger D, Pedersen D, Izquierdo S, Harriman R, Zhu L, Etches RJ, van de Lavoir MC, Harriman WD, Leighton PA. Chickens with humanized immunoglobulin genes generate antibodies with high affinity and broad epitope coverage to conserved targets. MAbs. (2018) 10(1):71-80.

• Könitzer JD, Pramanick S, Pan Q, Augustin R, Bandholtz S, Harriman W, Izquierdo S. Generation of a highly diverse panel of antagonistic chicken monoclonal antibodies against the GIP receptor. MAbs. (2017) 9(3):536-549.

• Mettler Izquierdo S, Varela S, Park M, Collarini E, Lu D, Pramanick S, Rucker J, Lopalco L, Etches R & Harriman W. High-efficiency antibody discovery achieved with multiplexed microscopy. Microscopy (Oxf). (2016) 65(4):341-52.

• Abdiche YN, Harriman R, Deng X, Yeung YA, Miles A, Morishige W, Boustany L, Zhu L, Mettler-Izquierdo S & Harriman W. Assessing kinetic and epitopic diversity across orthogonal monoclonal antibody generation platforms. MAbs. (2015) 8(2):264-77.

• Leighton PA, Schusser B, Yi H, Glanville J & Harriman W. A Diverse Repertoire of Human Immunoglobulin Variable Genes in a Chicken B Cell Line is Generated by Both Gene Conversion and Somatic Hypermutation. Front Immunol. (2015) 20;6:126

• Schusser B, Yi H, Collarini EJ, Izquierdo SM, Harriman WD, Etches RJ, Leighton PA. Harnessing gene conversion in chicken B cells to create a human antibody sequence repertoire. PLoS One. (2013) 21;8(11):e80108.

Engineering OmniChicken

• Collarini, E. J, Leighton PA, van de Lavoir M-C. Production of Transgenic Chickens Using Cultured Primordial Germ Cells and Gonocytes. Methods Mol Biol. (2019) 1874:403-430

• Dimitrov L, Pedersen D, Ching KH, Yi H, Collarini EJ, Izquierdo S, Van De Lavoir MC, Leighton PA. Germline Gene Editing in Chickens by Efficient CRISPR-Mediated Homologous Recombination in Primordial Germ cells. PLoS ONE, (2016) 11(4): e0154303.

• Leighton PA, Pedersen D, Ching K, Izquierdo S, Collarini EJ, Jacob R and van de Lavoir MC. Generation of chickens expressing Cre recombinase. Transgenic Research (2016) 25(5), 609–616.

• Schusser B, Collarini E, Pedersen D, Yi H, Ching K, Izquierdo S, Thoma T, Lettmann S, Kaspers B, Etches RJ, van de Lavoir MC, Harriman W, Leighton PA. 2016. Expression of heavy chain-only antibodies can support B-cell development in light chain knockout chickens. Eur J Immunol. (2016) 46(9):2137-48.

• Collarini E, Leighton P, Pedersen D, Harriman B, Jacob R, Mettler-Izquierdo S, Yi H, Van De Lavoir MC & Etches RJ. Inserting random and site-specific changes into the genome of chickens. Poult Sci. (2015) 94(4):799-803.

• Schusser B, Collarini EJ, Yi H, Izquierdo SM, Fesler J, Pedersen D, Klasing KC, Kaspers B, Harriman WD, van de Lavoir MC, Etches RJ & Leighton PA. Immunoglobulin knockout chickens via efficient homologous recombination in primordial germ cells. Proc Natl Acad Sci USA. (2013) 110(50):20170-5.

• US 2012/0090041 A1

• US 2015/0113668 A1

• US 8,703,485 B2

• US 8,907,157 B2

• US 8,030,095 B2

• US 8,415,173 B2

• US 8,592,644 B2

• US 8,865,462 B2

• US 9,380,769 B2

• US 9,394,372 B2

• US 9,404,125 B2

• US 9,549,538 B2

• US 9,644,178 B2

• EP 2 152 880 B1

• EP 2 336 329 B1

• EP 2 602 323 A1

• WO 2008/151081 A1

• WO 2008/151081 A8

• WO 2014/093908 A2

• OmniAb: Humanized Rodent and Chicken Platforms for Antibody Discovery

• OmniAb®

• OmniFlic®

• OmniMouse®

• OmniRat®

• OmniChicken™

• OmniClic™